VOMP3011 From URS to PQ: P&ID-1 Bioreactor Sketch and Performance Requirements Assignment (TU Dublin)

The P&ID-1 task in VOMP3011 is the starting point for the module’s validation project. You sketch a bioreactor with its jacket, agitator and bottom outlet pump, add every process and utility line from aeration and CIP to SIP condensate, rupture-disc venting and pH control, and then explain the key performance requirements a bioreactor must meet, which later feed your URS and PQ tests.

P&ID-1: Bioreactor

Step 1-1: Sketch the bioreactor P&ID

Sketch a basic P&ID for a bioreactor with the following main equipment components (your sketch can be hand-drawn or done using a basic software drawing package):

• Bioreactor vessel, with heating and cooling jacket
• Bioreactor agitator
• Bioreactor bottom outlet pump

Include the following process and utility connections on your P&ID:

1. Aeration supply to the bioreactor
2. Aeration vent/exhaust from the bioreactor with pressure control
3. CIP manifold supply to the bioreactor
4. Inoculum supply to the bioreactor
5. Media supply to the bioreactor
6. Clean steam supply to the bioreactor for SIP
7. Contaminated condensate (SIP) return
8. Process water supply and return distribution to and from the bioreactor
9. Over-pressurisation vent line to a catch tank via a rupture disc
10. Antifoam addition
11. Acid addition to the bioreactor for pH control
12. Base addition to the bioreactor for pH control
13. Bioreactor bottom outlet via pump to the harvest process manifold
14. Thermal-exchange fluid supply and return to and from the bioreactor’s heating and cooling jacket (from a temperature control skid)

Step 1-2: Performance requirements

List the primary performance requirements associated with a bioreactor. Write a sentence or two to expand on each of your performance requirements.